alexa 488

  • Alexa Fluor® 488 Protein Labeling Kit

     · Alexa Fluor® 488 Protein Labeling Kit 4 3.3 Calculate the degree of labeling moles dye per mole protein = A 494 × dilution factor where 71,000 cm-1M is the approximate molar extinction coefficient of the Alexa Fluor® 488 dye at 494 nm. For IgGs, we find that labeling with 4–9 moles of Alexa Fluor® 488

  • Alexa Fluor® 488 Phalloidin Cell Signaling

    Alexa Fluor ® 488 Phalloidin phalloidin 。

  • Alexa Fluor 488® Secondary AntibodiesJackson Immuno

    Alexa Fluor® 488 Technical Information Alexa Fluor® 488conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488.

  • Alexa Fluor 488 anti-mouse CD8a Antibody anti-CD8a53-6.7

    Alexa Fluor® 488 anti-mouse CD8a AntibodyCD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer.

  • Spectrum [Alexa Fluor 488] AAT Bioquest

    Alexa Fluor 488 spectrumAlexa Fluor 488 is a fluo excitation and emission wavelengths using the interactive Spectrum ViewerA web application for viewing and comparing spectra of various fluorescent compounds. Exportable as image or link.

  • Anti-Alexa Fluor 488, Rabbit IgG Fraction

     · tion of Alexa Fluor 488 dye, assayed in 100 mM sodium phos-phate, pH 8.0. Maximal quenching corresponds to more than 90% of the fluorescence of the free dye. Due to steric hindrance or limited accessibility of the Alexa Fluor 488 dye, maximal fluorescence quenching of Alexa Fluor 488 conjugates may be significantly less. Storage and Handling

  • SNAP-Surface® Alexa Fluor® 488 NEB

    488. SNAP-Surface ® Alexa Fluor ® 488 is a photostable green fluorescent substrate that can be used to label SNAP-tag ® fusion proteins in solution or on the surface of living cells. This is a cell impermeable substrate based on BG-Alexa Fluor 488. It is suitable for standard fluorescein filter sets. It has an excitation maximum at 496 nm

  • Alexa Fluor 488an overview ScienceDirect Topics

    Before use, Alexa 488 is dissolved at 50 m M in DMSO. 7. Alexa 488 solution is added to 2 mg/mL of F-actin at a final concentration of 240 μ M. It is recommended that DMSO be kept below 0.5% in the actin solution. 8. The mixture is stirred gently using a magnetic stirrer for 15 h at 4 °C in the dark.

  • Anti-rabbit IgG (H L), F(ab')2 Fragment (Alexa Fluor® 488

    Anti-rabbit IgG (H L), F (ab')2 Fragment (Alexa Fluor ® 488 Conjugate) was used as a secondary antibody. High content analysis of HeLa cells exposed to varying concentrations of staurosporine for 3hr. With increasing concentrations of staurosporine, a significant decrease (~2.5 fold) in phospho-MAPKAPK-2 signal as compared to the untreated

  • Alexa Fluor® 488 Mouse anti-Ki-67BD Biosciences

     · Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass

  • Alexa Fluor® 488 Mouse Anti-ERK1/2 (pT202/pY204)

    2 days ago · Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass

  • Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)

    Alexa Fluor® 488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor® 488 dye is water soluble and pH-insensitive from pH 4 to pH 10.

  • Alexa Fluor 488® Secondary AntibodiesJackson Immuno

    Alexa Fluor® 488conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green

  • Anti-mouse IgG (H L), F(ab')2 Fragment (Alexa Fluor® 488

    Anti-Mouse IgG (H L) F (ab') 2 Fragment antibody was conjugated to Alexa Fluor ® 488 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F (ab') 2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.

  • Alexa Fluor® 488-Streptavidin JIR

    Alexa Fluor® 488. Amax 493 Emax 519nm. Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures

  • Anti-rabbit IgG (H L), F(ab')2 Fragment (Alexa Fluor® 488

    Anti-rabbit IgG (H L), F (ab')2 Fragment (Alexa Fluor ® 488 Conjugate) was used as a secondary antibody. High content analysis of HeLa cells exposed to varying concentrations of staurosporine for 3hr. With increasing concentrations of staurosporine, a significant decrease (~2.5 fold) in phospho-MAPKAPK-2 signal as compared to the untreated

  • Alexa Fluor 488 dye Thermo Fisher ScientificUS

    Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range.

  • Anti-rat IgG (H L), (Alexa Fluor® 488 Conjugate) Cell

    Confocal immunofluorescent analysis of mouse hippocampus using F4/80 (BM8.1) Rat mAb #71299 detected with Anti-rat IgG (H L), (Alexa Fluor ® 488 Conjugate) #4416 (green) and Myelin Basic Protein (D8X4Q) XP ® Rabbit mAb #78896 detected with Anti-rabbit IgG (H L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) #4413 (red). Sections were mounted in ProLong ® Gold Antifade Reagent with DAPI

  • Spot-Label® Alexa Fluor® 488 Proteintech

     · Images were acquired with the Thermo Scientific CellInsight CX7, 20X objective. Left HeLa cells transiently expressing Spot-Tag®-Lamin B1 were immunostained with Spot-Label® Alexa Fluor® 488 (ebAF488, 1 800). Right Control staining of untransfected cells. Scale bar, 10 μm. Images were acquired with the Thermo Scientific CellInsight CX7

  • ALEXA FLUOR * 488 FLUORONANOGOLD™**

     · Alexa Fluor® FluoroNanogold™ is a unique, dual-purpose probe. Alexa Fluor® 488 FluoroNanogold™ reagents consist of affinity-purified Fab' fragments conjugated to both Alexa Fluor® 488 dye (green) and the 1.4 nm Nanogold® particle.1 An Alexa Fluor® 488 FluoroNanogold™ Fab’ conjugate is shown in Figure 1.

  • Alexa-488 C30H27N4O12S2 PubChem

    Alexa-488 C30H27N4O12S2 CID structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities

  • Alexa Fluor® 488 Phalloidin Cell Signaling Technology

    Alexa Fluor ® 488 Phalloidin allows researchers to fluorescently stain the cytoskeleton through the binding of phalloidin to F-actin. This product is intended for use on fixed and permeabilized samples due to the toxicity associated with phalloidin. After reconstitution the stock solution provides enough material to perform 300 assays based on

  • Results for "Alexa Fluor® 488" Abcam

    Description Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed. Application ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P. Reactivity Mouse. Conjugate Alexa Fluor® 488. (4).

  • Alexa Fluor® 488-conjugated antibodies Abcam

    Alexa Fluor ® 488 conjugated antibodies advantages compared to Fluorescein isothiocyanate (FITC) make it the most used green fluorophore for fluorescent cell staining. Comparable spectral properties and quantum yield. Brighter and more photostable when conjugated to antibodies.

  • Alexa Fluor® 488 annexin V/Dead Cell Apoptosis Kit with

     · Alexa Fluor® 488 annexin V/Dead Cell Apoptosis Kit with Alexa® Fluor 488 annexin V and PI for Flow Cytometry Catalog nos. V13241 and V13245 Introduction Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apoptosis is implicated in disease states, such as

  • Alexa Fluor® 488 Human Transferrin JIR

    Alexa Fluor® 488. Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing

  • Alexa Fluor® 488 Donkey Anti-Goat (IgG) secondary

     · Ab150129 Alexa Fluor® 488 donkey anti-goat IgG (H L) (shown in green) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Alexa Fluor® 488-Fab Donkey Anti-Goat IgG (H L)

    Alexa Fluor® 488. Amax 493 Emax 519nm. Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures

  • Alexa Fluor 488 anti-Cytokeratin pan reactive Antibody

    Preparation. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. Concentration. 0.5 mg/ml. Storage & Handling. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.

  • Alexa Fluor® 488 Goat Anti-Rabbit (IgG) secondary antibody

    The secondary antibody (green) was ab150077 Alexa Fluor ® 488 goat anti-rabbit IgG (H L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of

  • Alexa Fluor® 488 Protein Labeling Kit

     · Alexa Fluor® 488 Protein Labeling Kit 4 3.3 Calculate the degree of labeling moles dye per mole protein = A 494 × dilution factor where 71,000 cm-1M is the approximate molar extinction coefficient of the Alexa Fluor® 488 dye at 494 nm. For IgGs, we find that labeling with 4–9 moles of Alexa Fluor® 488

  • Alexa Fluor® 488 Protein Labeling Kit

     · Molecular Probes Alexa Fluor® 488 Protein Labeling Kit provides a convenient means to label proteins with our superior Alexa Fluor® 488 dye. The Alexa Fluor® 488 dye, which is spectrally similar to fluorescein, produces protein conjugates that are brighter and more photostable than fluorescein conjugates. In addition, unlike fluorescein, the fluorescence of the Alexa Fluor® 488 dye is insensitive to pH between pH 4 and 10. Alexa Fluor® 488

  • Alexa Fluor® 488 CD4 [EPR6855](Alexa

     ·  Alexa Fluor® 488 CD4 [EPR6855](Alexa Fluor® 488 Anti-CD4 antibody [EPR6855]) ,“”! 2016,(IVD),(POCT),

  • Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate

    This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 488 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the